2015-07-14 / Admin / 1483
Establishment of feeder-free culture system for human induced pluripotent stem cells on DAS nanocrystalline graphene

 The poster entitled “Establishment of feeder-free culture system for human induced pluripotent stem cells on DAS nanocrystalline graphene” was presented at the International Society for Stem Cell Research (ISSCR) 13th Annual meeting on June 24th, 2015, by prof. Jeong Beom Kim.

  Diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG) is a synthetic nanomaterial that has advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. DAS-NG based feeder-free and xeno-free culture method can maintain pluripotency of hPSC without biological extracellular matrix (ECM) coating. .Also, global gene expression analysis revealed, hPSC cultured on DAS-NG shared completely similar gene expression profile in pluripotency-related and focal adhesion-related genes with hPSC cultured on MEF. This culture platform can eliminate the concern of pathogen contamination from feeder or ECM, thereby facilitating clinical-grade hPSC generation.