[BME Colloquium] Translational Researches from Lab to Hospital :Rapid Antibiotic Susceptibility Test & High Throughput DNA Synthesis
  • SungHoon Kwon, Ph.D.(Seoul National University)
  • SLS Colloquia / May 15th 04:00pm / EB1-E104
Abstract
 

In this seminar, I will present two recent examples in my lab that successfully made translation from lab invention to hospital usage. 

1. Rapid Antibiotic Susceptibility Test  

A rapid antibiotic susceptibility test (RAST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs are not suitable for urgent cases of bacterial infection and antibiotic resistance due to their relatively long test time. I will present a fast AST method named single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. With SCMA, AST is finished only in 3 hours satisfying FDA guidelines. I will discuss application of SCMA to fast diagnostics of multi-drug resistant tuberculosis. 

2. High Throughput DNA Synthesis by Sniper Cloning: 

Writing DNA serves significant purposes in the fields of synthetic biology, functional genomics, and bioengineering. The megaclones on next generation sequencing (NGS) platform have potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. I will present an enabling technology named, ‘Sniper Cloning’, that enables the precise mapping of target clone features on NGS platform and noncontact rapid retrieval of targets for full utilization of megaclones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, we have lowered cost for DNA synthesis two orders of magnitude. I will present synthesis of shRNA library and target capture panel for NGS and their clinical utility.