[BME Colloquium]Applications of Fluorescence Imaging: From Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocytes
  • Jong Jin Kim, Ph.D. (Korea University)
  • SLS Colloquia / May 8th 04:00 pm / EB1 E104
Abstract
 

Congenital or acquired disorder in electro-mechanical properties of cardiac tissues is a serious health problem which can result in lethal cardiac arrhythmia and arrest. The mechanism through which pathological intervention leads to cardiac arrhythmia remains incompletely understood. Mapping cardiac electrical activity using fluorescence imaging techniques (optical mapping) continues to be at the forefront of innovations to better tackle new problems and to make significant advances to our understanding of cardiac arrhythmia mechanisms. Progress has been made in applying optical techniques to investigate the efficacy of stem cell therapies used to rescue infarcted hearts and to study normal and diseased human hearts. Furthermore, contributions from genetically encoded fluorescent probes have made optical mapping techniques even more versatile. Genetically encoded probes can be targeted to specific cells types in the heart or to cells implanted in the myocardium and thereby measure intracellular free Ca2+ (Cai) or membrane potential changes for weeks and even months, without loss of signal quality. The ability to track the survival of engrafted cells or engineered cardiac tissues and their coupling to the recipient heart for weeks after implantation provides critical data for the development of stem cell therapies.