[BIO Colloquium]Niches for the development of memory CD8 T cells in response to acute viral infections
  • Yong Woo Jung, Ph.D.(Korea University)
  • SLS Colloquia / Apr 8th 04:00 pm / AMRB 113

During viral infections, it is not well defined where within tissues effector and memory CD8 T cells form and persist. Our recent findings show that two subsets of effector CD8 T cells that have distinct cell fates are formed during acute infection ? the KLRG1hi IL-7Rlo short-lived effector cells (SLECs) and the KLRG1lo IL-7Rhi memory precursor effector cells (MPECs). Most of the SLECs undergo apoptosis after infection whereas most MPECs survive and become long-lived memory T cells. Here, we show that MPECs and SLECs are differentially localized within the spleen during LCMV infection. Using immunofluorescence microscopy, we demonstrate that the majority of MPECs were found in the T cell zone, while SLECs were exclusively localized in the red pulp during the course of an immune response to LCMV infection. MPECs homed to the T cell zone using pertussis toxin-sensitive chemokine receptors, and appeared to contact with gp38+ stromal cells, which produce chemokines CCL19 and CCL21 and the T cell survival cytokine IL-7. In addition, these MPECs were aggregated in the specific microenvironment of the liver. These data suggest that the preferential survival of the MPECs and the memory T cells may be tightly linked to their anatomic localization in the spleen and liver.